61P Biomarkers EGFR, p53, IDH1 and MDM2 as prognostic indicators for overall survival of glioblastoma patients

Glioblastoma (GBM) is one of the deadliest cranial tumors occurring in adults. Various biomarkers have been tested for their significance diagnosis, prognosis, and treatment GBM. Some well-studied markers GBM are Isocitrate dehydrogenase1 (IDH1), Murine double minute2 (MDM2), Epidermal Growth Factor Receptor (EGFR) p53. The aim this study was to investigate protein expression these patients Pakistan. A total 51 surgically resected formalin-fixed paraffin-embedded specimens from diagnosed treated at Aga Khan University Hospital were included study. Immunohistochemistry (IHC) IDH1, MDM2, EGFR p53 performed using Dako EnVision System respective monoclonal antibodies. Survival analysis check association with prognosis patients. There 36 males 15 females study, a median age 48 time diagnosis. Overexpression molecular as follows: 55% EGFR, 22% p53, 79% IDH1 85% MDM2. We did observe that significantly associated increased our worse survival. Age >40 years predictor well. overexpression advanced prognostic indicators.